Visit your Premier League club's page with all the latest news, analysis and fan views, and sign up for notificationsĪll matches kick off at 15:00 GMT unless otherwise stated.Premier League - week 16Ī correct result (picking a win, draw or defeat) is worth 10 points. What needs to happen is for the takeover to go through and for Ratcliffe to take control of the footballing affairs - let's see how Ten Hag does then."Ĭhris Sutton and Dougray Scott were speaking to BBC Sport's Chris Bevan. "That's why United have been struggling, not Ten Hag - I think he is a terrific manager. "They have been destabilised for so long because, despite potentially being the biggest club in the world, their financial model under the Glazers means huge sums of money are being taken out of the club and they are piling debt upon debt upon debt. "At the moment we are waiting to see whether Sir Jim Ratcliffe will complete a deal for 25% of the club but, for years, the problem at United has been from the top down. He has not become a bad manager overnight, and I'm actually surprised he has done as well as he has done, considering what is going on behind the scenes. "I've read all this stuff about Erik ten Hag losing the dressing room but I don't believe that. We have had some great teams down the years, but we are just not very good at the moment. He explained: "I've followed them since I was a boy, again because of my dad. Scott has an English team too - Manchester United - just like his father. It's obviously not at the same level as the Premier League, but I watch Hibs every week and there is still a lot of good passing football being played despite it being a very physical league." "I can't believe Sutton has gone for Bournemouth to draw with Manchester United!" Suranne Jones, who plays detective Amy Silva, shares some big news with Scott / Grainger during series two of Vigil "Scottish football gets a bad press but I think it is a really good standard. He didn't seem to have an affinity with Scottish football. "We are doing OK at the moment under Nick Montgomery, but under Lee Johnson we were terrible. I was there and it was just phenomenal - our 114-year wait to win the cup was finally over, and it was a fantastic day. "My favourite moment has to be the 2016 Scottish Cup final when we beat Rangers 3-2 at Hampden. We were fanatical - I still am, I have to say - it pains me when we get beat! "I started going with him in the 1970s and we used to go home and away together. "So he was watching Hibs in the 1950s with 'The Famous Five' - our legendary frontline of Gordon Smith, Bobby Johnstone, Lawrie Reilly, Eddie Turnbull and Willie Ormond - who were part of such a great team. "My dad lived in Glasgow as a boy but became a mad Hibee because his uncle was a scout for Hibs and he used to take him to Easter Road," Scott told BBC Sport. Scott followed in his father's footsteps and grew up a staunch Hibernian fan. Series one was set on a submarine, but series two takes to the skies and the secret world of drone warfare Scott plays air vice-marshall Marcus Grainger in series two of Vigil. Season two of Vigil begins on Sunday, 10 December at 21:00 GMT on BBC One, with the first three episodes available on BBC iPlayer from 06:00 that morning. Sutton is making predictions for all 380 Premier League matches again this season, against a variety of guests.įor this weekend's games, he takes on actor Dougray Scott, who stars in the new season of high-stakes drama Vigil. The question is, can they go again and play at that level against the table-toppers? It's going to be hard for Villa to replicate that."
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A precision engineered Apex Predator: Mechagodzilla!ĭon its armor and become the apex hunter you were born to be with the Tracer Pack: Mechagodzilla Limited Time Bundle. In a time of monsters, humanity built its own… designed to make us the ultimate dominant species. Solution – Pour the transfer buffer slowly into your tank to keep bubbling at a minimum.Pouring transfer buffer into transfer apparatus too quickly.Solution – Mix fresh buffer without shaking – use a stir bar.Solution – Degas your transfer buffer.The agitation of mixing transfer buffer causes bubbling that can then get trapped. This allows me to use the rest of the blot without worry. Then when I use the blot later and the Ponceau S is washed away during blocking and incubation steps, I will still be able to see the compromised portion of the blot. Usually bubbling is minor, and if it is just a couple of bubbles like in the blot above, then I simply use a gel pen to circle them on the blot. Bubbles are the result of air being trapped between the membrane and the gel during transfer. A bubble appears as a white spot on your membrane in the middle of the field of red Ponceau S stain. Watch our easy-to-follow video protocols.Bubbling can happen for many reasons.Protocols are provided by Abcam “AS-IS” based on experimentation in Abcam’s labs using Abcam’s reagents and products your results from using protocols outside of these conditions may vary. 4 g NaCl (500 mL distilled water) pH 7.4 Primary antibody made up in TBS with 1% BSAĮxample is of primary antibody used at a dilution of 1:10.Įxample is of ABC, each part used at a dilution of 1:100.īicarbonate/carbonate coating buffer (100 mM)Ħ.0 g NaHCO 3 (1 L distilled water) pH 9.6 Nuclear fractionation protocol reagents buffer B Nuclear fractionation protocol reagents buffer A Add 0.8 mL β-mercaptoethanol under the fume hood Bring volume up to 1 L with distilled water.Adjust the volume to 800 mL with ultra pure water.TBST (Tris-buffered saline, 0.1% Tween 20) The final molar concentrations of the 1x solution are 20 mM Tris and 150 mM NaCl.For a 1x solution, mix 1 part 10x with 9 parts distilled water and pH to 7.6 again.Add distilled water to a final volume of 1 L.If too basic, adjust to pH 7.6 with concentrated HCl, and if too acidic, adjust with concentrated NaOH. The pH of the solution should be about 7.6 at room temperature.TBS 10x alternative recipe (concentrated Tris-buffered saline)ĥ.6 g Tris base (formula weight: 121.1 g) This avoids the large volume of potentially hazardous hydrochloric acid that is needed to neutralize a solution of Tris base alone. The final molar concentrations of the 1x solution are 20 mM Tris and 150 mM NaCl.Īn alternative recipe for Tris buffer combines Tris base and Tris-HCl. TBS 10x (concentrated Tris-buffered saline)Īdd distilled water to a final volume of 1 Lįor a 1x solution, mix 1 part of the 10x solution with 9 parts distilled water and adjust pH to 7.6 again. Bring up to the initial volume with waterĪvoid large changes in volume during boiling put a loose lid on the container to protect from evaporation.Repeat this cycle until the solution remains at pH 9.0 after boiling and cooling.Prepare a 100 mM sodium orthovanadate solution with double distilled water.Failure to filter can lead to spotting, where tiny dark grains will contaminate the blot during color development.Īll procedures must be carried out under the fume hood. 3–5% milk or BSA (bovine serum albumin).For proteins >80 kDa, we recommend including SDS at a final concentration of 0.1%. Loading, running, transfer, and blocking buffers 1.0% NP-40 (possible to substitute with 0.1% Triton X-100)Ĭytoskeletal bound proteins extract buffer.The 10% sodium deoxycholate stock solution (5 g into 50 mL) must be protected from light. It can also disrupt protein-protein interactions and may, therefore, be problematic for immunoprecipitations and pull-down assays. A RIPA buffer gives low background but can denature kinases. RIPA buffer (radioimmunoprecipitation assay buffer) RIPA buffer contains the ionic detergent sodium deoxycholate as an active constituent and is particularly useful for nuclear membrane disruption for nuclear extracts. Lysis buffers These buffers may be stored at 4☌ for several weeks or aliquoted and stored at -20☌ for up to a year. You will be prompted for a two-step authentication.Full Name: the name you want displayed on your outgoing mail.Otherwise, go to the Mail menu and click Add Account > Exchange and then click Continue.If you have not previously configured an account in Mail you will be prompted to add an account.Launch Mail. (Click the icon in the dock or go to the Applications folder to open it.).If you have trouble with your email using the Apple mail client, follow the guide below:. Once the account has been added successfully you should be prompted with Duo.Select Manage Accounts, then add account.To resolve this issue whilst using the Mail App for Windows, you will need to remove and re-add your email account. Enter your Northumbria Username and Password.To add the account, navigate back to File.Select the account you wish to remove, then click remove.Select Account Settings from the Account Settings dropdown.From the main Outlook view, select File in the upper left of the screen.If you are having issues using the Outlook app, you will first need to remove your account and re-add it. If you are not receiving emails or are struggling to log into your Northumbria email account you will need to remove your account and re-add it, below are supported platforms and their solutions to fix these issues. Applicant Terms and Conditions and Policies.International Fees, Funding & Scholarships.
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The rear Currie RockJock 44 style axle features a centered differential housing and equal length chromoly axle shafts, with the same gears and spool found in the front axle.Ī machined aluminum 3-gear Vanquish transmission case houses Incision steel gears, and an updated motor plate with integrated standoffs provides a more secure motor mount. The VS4-10 drivetrain is based off our proven parts you already know and trust. The licensed Currie Enterprises RockJock 44 style front axle features an offset front differential housing and uses chromoly VDI universal axle shafts, high-pinion hypoid ring gear, chromoly 6 bolt spool and patent pending one-piece bearing retainers to create an incredibly scale and durable front axle assembly. Standard Chassis Bumper Mounting Geometry.Optional Rear Upper Link Mounting Locations.Aluminum Rock Sliders with plastic lower skid plates.Low Center-of-Gravity Battery and Electronics Tray.Beyond the aluminum sliders the plastic lower slider plates allow a smooth transition from the skid to the edge of the slider. Full width aluminum front and rear bumpers are durable and lightened, and when combined with the aluminum rock sliders that capture the sides of the Origin body you have the armor needed for maximum protection. The one-piece aluminum battery and electronics tray hold the electronics low in the chassis and help to maintain a forward weight bias for increased stability and control. Molded shock towers capture the top of the 90mm Incision Scale Shocks in double sheer for maximum support. The chassis allows clearance for both brushed and brushless 540 motors, and a transmission base spacer is included to provide rear upper link clearance at the motor if needed. The panhard mount on the chassis is made from machined aluminum to withstand the impacts of severe trail conditions. Full width molded front and rear bumpers increase approach and departure angles and allow for flexibility during impacts on the trail.The VS4-10 Chassis is built to provide a scale platform that doesn't hold back on performance. The chassis arches provide plenty of room for ride height adjustments, with allowance for suspension up-travel and clearance around the pumpkins. The aluminum battery and electronics trays provide multiple locations for battery and electronics placement. Molded shock towers capture the top of the 80mm Incision Scale Shocks in double shear for maximum support. The VS4-10 chassis is developed to work with the included Vanquish Products VFD Spec transmission. The molded rock sliders come with a replaceable outer portion that perfectly fits the Origin Halfcab body. The steel C-channel design provides scale detail, molded front and rear cross members fill the chassis rails in the highest stress locations for ultimate strength and provide features like dual servo mounts, winch line routing, and a fuel cell. Vanquish Products VS4-10 Chassis is made to provide you with a balance scale looks and unhindered performance. Translite Global's TL-MC84 MoCA 2.5 Network Adapter is designed for data transmission over coaxial cable. as well as moving the "PoE" MoCA filter to the input of the top-level splitter, to maximize the performance benefit of the filter.Translite Global TL-MC84 MoCA 2.5 with 2 Gigabit Ethernet Ports - Ethernet Over Coaxial Adapter (2 Pack) Not that it's certainly your issue, but I'd look at replacing both splitters with known-good "designed for MoCA 2.x" splitters*. If you’ll be supplanting the G1100’s built-in MoCA LAN bridge with a standalone adapter, you’ll need to disable the MoCA LAN bridge via the G1100’s configuration UI or disconnect the G1100 from the coax, though the latter can only be done if the G1100’s WAN link with the ONT is over Cat*/Ethernet. here’s a listing of available MoCA adapters: Thanks so much for making this clear! I went ahead and ordered 2 of the MOCA 2.0 bonded adapters to increase the throughput.įYI. either matching the bonded MoCA 2.0 spec of the MM1000 (800-1000 Mbps) or bumping both adapters up to MoCA 2.5 (up to 2000-2500 Mbps shared). If greater throughput is required, you’d want to supplant the G1100’s built-in MoCA bridge with a separate, standalone MoCA adapter at the G1100. Theoretically, yes, but some due diligence would be required to verify that the coax outlets interconnect.Īssuming connectivity, the G1100 sports a standard MoCA 2.0 LAN bridge, so could achieve up to 400-500 Mbps throughput with a bonded MoCA 2.0 Motorola MM1000 adapter. And though the BAMF product description may make the claim, it also says it's optimized for satellite - and it can't be both. Neither of these splitters are optimized for MoCA. I did look at the redit link but i got confused lol. Internet speeds via wired lan are between 800-950 mbps. but i disconnected it i seemed to be getting ip conflicts.Īll the devices in the junction run to different rooms but only are connected to 2 STB one off the splitter and one homerun connecton all other coax is open ended. Yes the switch is coming off of one of the routers lan ports. Yes a set STB is hanging off that splitter. Not sure how you would do that but if it needed to be done i have more then enough cat6 to do itĮverything is that picture is in the same room except the ont and junction box. Then the coax on the splitter is coming from the junction box then being split off to the router and tv. fiber into the house / coax in the junction box and back in. it seems the ont is feeding the cable junction box outside. The splitter "optimization" is secondary to the diagram posted, which I can use some help understanding:Ĭlick to expand.thank you so much for taking the time to help me. The diagram would benefit by providing more detail on the central junction, as well as how the pictured components connect to the central junction and illustrating device co-location. Is this switch connected to the router's LAN? (It would need to be.) (MoCA adapters use a diplexer, internally, to redirect the frequencies where needed, rather than using a simple splitter, so the "IN/OUT" port on the Translite is designed to pass frequencies up to 1002 MHz.)Ģ) The diagram indicates that the MoCA adapter is connected to a switch. ! Critically, if the router's WAN connection is via MoCA or a FiOS set-top box is hanging off that 2-way splitter, they MUST NOT be connected via the MoCA adapter's pass-through port, as the "IN/OUT" pass-through port severely attenuates signals at MoCA frequencies. ? Are the devices pictured in the same room/location? ? Are you also subscribed to FiOS TV service? ? Can the router be connected via Ethernet to the ONT? ? What is your ONT's WAN connection type, Ethernet WAN or MoCA WAN? ? What is the brand/model # of the router? And that you have a TV connected downstream of the MoCA adapter, as well. 1) The diagram appears to show the router ONLY connected via coax, via the MoCA adapter's pass-through port. Indeed, you will use it to highlight an element, and what would be better than being able to blur a background, for example? On a 35mm, the interest is however much more present. On a 20mm wide angle lens, especially if you use it for landscapes like I do (so essentially between f/5.6 and f/11), the interest of having a large aperture is really arguable. Then, it will be up to you to determine the interest you have to own a 35mm lens with a larger or smaller aperture. Since you can capture twice as much light, you will be able, if you wish, to reduce your ISO by 2, allowing you to keep a better image quality.This is why lenses with large apertures are also called “fast lenses”, Therefore, you will be able photographing twice as fast. Remember that every time you open an aperture (e.g., from f/4 to f/2.8), you will double the amount of light reaching the sensor. Get more blur in your images (either in the foreground or in the background),.Having a larger aperture will essentially allow you to do three things: The smaller the number, the more light the lens will be able to collect (and vice versa). As a reminder, the aperture is the number behind the “f/” on your lens. The maximum aperture of your lens is the first thing to look at closely, which should guide you in your choice.Īs far as I know, you can find apertures from f/1.2 to f/2.8. Knowing that it is a prime lens, no need to talk about the choice of your focal length here. The article is therefore more adapted to people with a full format camera, or even APS-C, keeping in mind that a Sony 35mm mounted on your camera, will give a longer view. For an equivalent field of view for a Micro 4/3 sensor (Panasonic and Olympus cameras), you will have to look at 17mm lenses. If you own a Sony APS-C camera (type A6000 to A6600), you can have a look at our article on the best 24mm Sony lenses, which is equivalent to a 35mm angle of view on a full frame camera. In this article, I decided not to make the distinction between lenses dedicated to APS-C sensors and those dedicated to full-frame. It is equivalent to a standard focal length, even though the crop factor on an APS-C sensor will give you a focal length equivalent to 50mm on full-frame. Many photographers consider the 35mm as the “all-purpose” focal length. Now that I already told you about wide-angle and ultra-wide-angle lenses from the brand, I decided to write this article on the best 35mm lenses for Sony mirrorless cameras. After writing a lot of generic articles about cameras and lenses, I’m taking it a step further by trying to help you choose a specific lens, whether it’s a telephoto lens, a wide-angle lens, or a macro lens. 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